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You don't know where acid stops and reality starts.
The formic acid stops metabolism, lyses cells, and stabilizes small, phosphorylated compounds (19).
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TMB-Ultra (Pierce, Rockford, IL) was used for color development with sulfuric acid stop solution and absorbances read on a microplate reader (BioRad, Hercules, CA).
The color development is stopped with acid stop solution.
The reaction was stopped by the addition of a Tris (pH 8)/ethylenediaminetetraacetic acid stop mix.
The color reaction was terminated by the addition of a sulfuric acid stop-solution.
A horseradish peroxidase conjugate bound to the detection antibody and colour development was accomplished by the addition of tetramethylbenzidine substrate and stopped with an acid stop solution.
The application of formic acid stopped listeria growth efficiently at DM 200 g/kg, but not at DM 430 g/kg.
To investigate contact activation on different surfaces, the degree of plasma kallikrein expression (Chromogenix) present on surface-exposed plasma was evaluated with an acid stop method.
After that, these samples were washed and immediately added with 3,3′,5,5″-tetramethylbenzidine 3,3′,5,5″-tetramethylbenzidinelowed by 1 M Phosphoric acid sTMB solution.
To calculate comparable estimates of protein identity all positions containing at least one gap, unknown amino acid, stop codon or mis-translated regions caused by frameshifts were excluded.
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