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TMB-Ultra (Pierce, Rockford, IL) was used for color development with sulfuric acid stop solution and absorbances read on a microplate reader (BioRad, Hercules, CA).
The color development is stopped with acid stop solution.
The reaction was stopped by the addition of a Tris (pH 8)/ethylenediaminetetraacetic acid stop mix.
To investigate contact activation on different surfaces, the degree of plasma kallikrein expression (Chromogenix) present on surface-exposed plasma was evaluated with an acid stop method.
A horseradish peroxidase conjugate bound to the detection antibody and colour development was accomplished by the addition of tetramethylbenzidine substrate and stopped with an acid stop solution.
After that, these samples were washed and immediately added with 3,3′,5,5″-tetramethylbenzidine 3,3′,5,5″-tetramethylbenzidinelowed by 1 M Phosphoric acid sTMB solution.
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You don't know where acid stops and reality starts.
The color reaction was terminated by the addition of a sulfuric acid stop-solution.
The formic acid stops metabolism, lyses cells, and stabilizes small, phosphorylated compounds (19).
The application of formic acid stopped listeria growth efficiently at DM 200 g/kg, but not at DM 430 g/kg.
In the past the procedure was typically performed by a local woman using anything from a razor blade to a piece of broken glass, sometimes even using battery acid to stop the bleeding.
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