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Deduced amino acid sequences were aligned using the multiple alignment ClustalW2 program (http://www.ebi.ac.uk/Tools/msa/clustalw2).ac.uk/Tools/msa/clustalw2
Amino acid sequences were aligned using the MAFFT alignment tool plug-in in Geneious Pro 5.0.4 (BLOSUM72, gap open penalty: 1.53, offset value: 0.123, E-INS-i settings).
The identified amino acid sequences were aligned using the ClustalWS sequence alignment program with standard settings (Gonnet weight matrix, gap opening penalty 10.0 and gap extension penalty 0.20) through the JABAWS 2 tool in Jalview 2.7 [ 47].
Reference and novel nucleotide, and corresponding amino acid sequences were aligned using the Geneious 4.7 sequence alignment tool and editor [ 53].
For each family, amino acid sequences were aligned using the MAFFT (v7.149) multiple sequence alignment algorithm using the E-INS-i strategy with the default offset value (0) for OBPs as they have multiple large gaps, and the G-INS-i strategy with an offset value of 0.123 for CSPs as they largely share global homology and are all about the same length [ 70].
Amino acid sequences were aligned using the ClustalX program (version 1.81; multiple alignment parameters: gap opening 10, gap extension 0.20; DNA weight matrix: IUB; Protein weight matrix: Gonnet series) (T hompson et al. 1994).
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The translated amino-acid sequences were aligned using the program FFT-NS-I nested in Mafft version 5 [ 42].
Amino acid sequences were aligned using MAFFT 7.164b with the L-INS-i option.
HA nucleotide and amino acid sequences were aligned using MUSCLE with two alignment iterations [65], [66].
Multiple alignments of amino acid sequences were aligned using Clustal X.
The amino acid sequences were aligned using MUSCLE [ 34].
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