Use one 24-deep-well plate for each amino acid position.
Top panel, HLA-C*0602; middle panel, amino acid position 67 in HLA-B; bottom panel, HLA-A*0201.
a, Alignment of C-terminal TLR-4 protein sequences from different primate species (starting at human TLR-4 amino acid position 821).
Furthermore, no convincing evidence for association was observed for the other reported ACPA− RA association to HLA_DRB1*03 (P=0.36, amino acid position 11; P=0.02).
Numbers above indicate amino acid position based on the sequence of human BubR1 and B56γ1.
Numbers above indicate amino acid position based on the sequence of human B56γ1.
The amino acid position mutation matrix TM×L is constructed as follows.
Fig. 5 shows the curve shapes of amino acid position mutative equation (Eq.7).
The conservation scores at each amino acid position were calculated using the same server.
First, a conserved leucine (L) is absent at amino acid position 482.
Before computing the amino acid position mutation correlation matrix, we have to build the amino acid position mutative equation, which measures the mutation of amino acid k at position l in protein family.
