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The reaction was then concentrated under reduced pressure and purified by reverse-phase HPLC (35 80% gradient acetonitrile in acidic water (0.1% trifluoromethanesulfonic acid), monitoring product elution at 230 nm) to provide product 17b (0.037 g, 65% yield).
A mass loss/consumed acid monitoring technique has been also developed in order to assess monolithic samples.
The objective of the present study was to evaluate the accuracy of EasyTouch uric acid monitoring system.
The large majority of catabolic illnesses were managed successfully at home using 'sick-day' formula and frequent amino acid monitoring.
To clarify further the specificity and sensitivity of serum sialic acid monitoring, analyses of sialic acid by the thiobarbituric acid method and acute-phase reactants by radial immunodiffusion were made using the same malignant melanoma patients' sera.
Using esophageal acid monitoring, one of the most sensitive and specific techniques used to diagnose acid regurgitation, we conducted a physiological study of patients with CAP following a lobectomy procedure.
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In the microbiological test (pour plate method), use was made of the test bacteria Pseudomonas aeruginosa, while the photochemical test involved the degradation of para-chlorobenzoic acid, monitored by HPLC method.
Citrate synthase: Citrate synthase activity was determined as the reduction of 5, 5′-dithiobis-2-nitrobenzoic acid, monitored at 412 nm in reaction buffer (100 m m TrisHCl, 50 μ m Acetyl-CoA, 0.1 m m DTNB, 0.1% Triton-X, 0.25 m m oxaloacetate, pH 7.4).
Precipitated material was removed by centrifugation and formation of L-[1-C]galactonic acid monitored by HPLC on a Metacarb 87C 300 × 7.8 mm column (MetaChem Technologies Inc., Torrance, CA) maintained at 70°C with a mobile phase of ultrapure water flowing at 1 ml min-1.
Continued long-term, standardized monitoring of vertebrate body condition should be paired with concurrent direct (stomach contents) or indirect (isotopes, fatty acids) monitoring of diet, detailed study of movements and seasonal ranges to establish and refine baselines, and identification of critical habitats of the marine vertebrates being monitored.
These extreme increases were not influenced by supplementation with pSer and had no dramatic effects on the canonical amino acids monitored in our experiments.
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