Six amino acids encoding for the epitope 78 83 (DPASRE) of the hepatitis B core (HBc) antigen were introduced within the different loops of the L1 protein at positions 56/57, 140/141, 179/180, 266/267, 283/284 or 352/353.
This included an upstream terminator region, SOS-box, sigma-factor binding site, ribosomal binding site, and the first 29 amino-acids encoding for colicin E2.
We also examined this relationship for subgroups of trinucleotide motifs defined by the degeneracy of amino acid encoding, i.e., for 2fold-, 4fold-, and 6fold-degenerate codons.
To date, all the reported CUL3 PHA2E mutations are heterozygous, transmit as autosomal dominant traits and result in the deletion of the amino acids encoded for by exon 9 of the CUL3 mRNA (residues 403 459) (Boyden et al, 2012; Grimm et al, 2012; Osawa et al, 2013; Tsuji et al, 2013; Glover et al, 2014).
Strong differences in amino acid abundance are also observed for amino acids encoded by quartet codons, such as Ala-GCN (3.9% ± 1.4%) and Val-GTN (9.7% ± 3.1%), and some amino acid encoded by AT-rich codons (Asn-AAY, Lys-AAR, and Ile-ATY).
The deduced amino acid sequence encoding for the plant defensin peptide was aligned against sequences of defensin holotype type 1 (Raphanus sativus Rs-AFP1, GenBank: AAA69541.1) and defensin type 2 (Nicotiana alata NaD1, GenBank: AAN70999.1) using ClustalX [ 22].
The ModA amino acid sequence (encoding for the periplasmic Mo-binding protein of the ModABC transport system) from A. variabilis was aligned with ModA sequences from a variety of microbial species and used to calculate a distance-based phylogenetic tree (Fig. 1).
The S. pyogenes open reading frame (amino acids 42 484) encoding for the M1 protein (UniProt ID: Q99XV0, emm1) was cloned at the Lund Protein Production Platform (LP3) (Lund, Sweden).
Of the exonic SNPs, only two, in exon 4, are non-synonymous SNPs leading to altered amino-acids (SNP12063 encoding for codon 47 and SNP12139 coding for codon 72) [15].
The nucleic acid encodes the genetic information unique for each virus.
A recombinant human adenovirus serotype 5 containing an expression cassette for human soluble CD59 (sCD59), which lacks the C-terminal 26 amino acids encoding the signal sequence for attachment of the GPI anchor, was generated (AdCAGsCD59, Figure 1A) essentially as described by us previously [20].
