Exact(9)
In contrast, deletion of both sites N and A increases DNA methylation in both regions, as indicated by successful amplification of these regions after digestion with the restriction enzyme Aci I.
The restriction enzyme Aci I recognizes the sequence 5'-GCGG-3'.
Aci I digested 3'-anchored cDNA libraries were constructed as previously described [ 40, 41].
There are 18 Aci I sites and 65 CpGs in the TSC2 promoter.
The absence of any digestion suggested that the Aci I digestion of the samples was specific (data not shown).
Approximately 500 600 ng of pooled and gel purified PCR product was digested with Aci I at 37°C for 6 hr.
Similar(51)
Two such Type II strain tag sequences were selected for this analysis: (1) the SUL1 (MnI) SAGE tag, CATGACATCGAGGA (present at nucleotide position 332,392 in genomic contig TGG_994387), and (2) the AcI I-SNP tag CATGCTCCGCTACA (nucleotide position 39,009, contig TGG_994300).
On Valentine's Day itself, Vassily Petrenko conducts mezzo-soprano Jane Irwin and the Royal Liverpool Philharmonic in Chausson's intoxicating Poèmes de l'amour et de la mer at the Philharmonic Hall, Liverpool (0151-709 3789), while the Gabrieli Consort perform Handel's Aci, Galatea e Polifemo and Acis and Galatea at the Wigmore Hall, London (020-7935 2141).
I tasted one of the first bottles ever made from the aci kara ("bitter-black") grape, a rare indigenous varietal that is being grown in the foothills above the Mediterranean — no matter that the tannin made it almost impossible to swallow.
With a plot derived from Ovid's "Metamorphoses," the infrequently performed "Aci, Galatea e Polifemo" tells the story of the lovers Galatea and Acis, the latter killed by the jealous Cyclops Polyphemus.
One of the good things about "Aci" was that, vocally, Costanzo was merely first among equals.
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