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On this consideration, the quenching mechanisms are studied and the processes are controlled by multiple mechanisms in which dynamic and static mechanisms are calculated, achieving the quantification evaluation of the quenching process.
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As the approach has now been validated, future work will continue to concentrate on streamlining the technology for use in a clinical setting and achieving the most accurate quantification of suspicious lesions as possible.
In some cases, low-resolution mass spectrometers may not achieve the required quantification limits and more sensitive detection may be required.
Irrespective of the polarity mode of the acquisition method used, alkaline conditions achieved the best quantification limits and linear dynamic ranges.
It is impossible to achieve the best quantification and minimum crosstalk using only the early gates because of the comparable contribution of the two fluorophores at the early time points (Fig. 3).
By achieving the low limit of quantification (0.4 ng/mL for saliva and 0.8 ng/mL for urine), the presented method can be useful to assess the exposure to tobacco smoke as ETS and active smoking.
Another reason for not further pursuing clean-up in LC MS MS analysis was that the sensitivity of current triple-quadrupole instruments enables injection of only small amounts of matrix into the LC MS MS system (e.g. 2 mg) while still achieving the desired limits of quantification.
It is worth mentioning that these features were achieved by the quantification of the amount of antigen through the precise identification of cell contours in individual cells.
The directional knowledge should be coupled with the sub-vectors' norms quantification to achieve the full multiplexing gain.
Air-drying or fixing cells with glutaraldehyde does not achieve the same sensitivity and accurate quantification.
In [ 10], the method requires translation of both the sample and reference arms, and must also sacrifice spatial resolution to achieve the spectral resolution required for quantification.
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