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This chapter focuses on control and protection systems for achieving site maximum safety and reliability.
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In this protocol, we provide detailed, step-by-step instructions for generating new hybrid recombinases with user-defined specificity, as well as methods for achieving site-specific integration into targeted genomic loci using these systems.
This realization led several groups to contemplate the potential benefits of achieving site-specific conjugation of drug to antibody, wherein both the modification sites and DARs could be more precisely controlled.
We focused our design on recognition of distance and geometry as the simplest parameters to achieve site selectivity, which has allowed us to activate CH bonds at remote sites that are previously not accessible.
Drug nanocarriers are often derivatized with targeting moieties to achieve site specific delivery, however, the results from this approach have, as yet, not reached expectations.
To achieve site specificity, Lf was used as a ligand in present project.
Abundance and species richness from the three samples within the site were pooled to achieve site level data.
While these glycoconjugation methods have provided a straightforward means to achieve site specificity, one downside is the lack of control over site placement.
In the CRISPR/Cas9 system, because a ~20 nucleotide target sequence is used to achieve site-specific DSB, these difficulties can be avoided in some cases.
Temperature-sensitive liposomes are considered to be a promising tool to achieve site-specific delivery of drugs.
In this chapter, we have explored the strategies and advances made toward the development of polymeric nanocarriers in order to achieve site-specific gene delivery.
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