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In this report, we describe the use of the P. aeruginosa T3SS as an alternative method to deliver functional Cre recombinase to the nuclei of differentiated and pluripotent cells, achieving DNA recombination through loxP sites on the chromosome, resulting in alteration of host cell gene expression.
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Artemis, in turn, is responsible for DNA end processing in order to achieve DNA end structures suitable for ligation.
Although gene ablation in T cells with DNA nucleofection of CRISPR reagents was also achieved, DNA nucleofection is associated with high toxicity to T cells, which represents a major difficulty for its application (Mandal et al., 2014; Su et al., 2016).
To achieve DNA hybridization, the DNA-modified channel was exposed to NC and PC solutions for 5 h at 30°C.
In order to achieve DNA cyclization, we designed two ss-DNA probes: the leftpart probe could form a "hairpin" structure by denaturing; the rightpart probe could also form a "hairpin" structure based on analyte-activated conformation change.
Typically, a specific DNA donor strain is required to achieve DNA transfer in conjugation.
Cells were exposed to 10 μM 5-aza-2′deoxycytidine (AZA; Sigma, St . Louis MO) for 96 hours to achieve DNA demethylation, which constituted our epigenetic modification treatment.
It is thus possible to achieve DNA methylation loss through a C to U deamination and subsequent replacement of adjacent methylated cytosines by LP BER.
DNA protein conjugates are necessary to achieve DNA-directed protein assembly for the fabrication of multienzyme complexes on DNA scaffolds.
TCF7L1 is known to form a complex with LEF1 to achieve DNA-binding ability.
Mutations occurred predominantly in those strains sensitive to frame-shifting mutagens (Brown and Dietrich, 1979), which suggest that anthranoids might achieve DNA-damage probably by intercalation.
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