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MMP identity and normalization between gels was achieved with human recombinant MMP-2 (PF037, Calbiochem) and MMP-9 (PF038, Calbiochem).
Glargine treatment resulted in phosphorylation levels of IR and Akt that were comparable with those achieved with human insulin, although delayed in time in some tissues.
The challenge for hypoxia imaging is to make images showing low levels of tissue pO 2 demonstrating a phenomenon that occurs at a much smaller scale than can be achieved with human imaging techniques (1 5 mm resolution).
Peak IR and Akt phosphorylation levels induced by insulin glargine were generally comparable with those achieved with human insulin, although in some tissues the effects of insulin glargine were delayed and (or) prolonged in time.
Therefore, the challenge for hypoxia imaging is to measure low levels of tissue pO2 on a spatial scale similar to the O2-diffusion distance (70 100 μm); a much smaller dimension than can be achieved with human imaging techniques.
Therefore, the challenge for hypoxia imaging is to measure low levels of tissue pO 2 on a spatial scale similar to the O 2 diffusion distance (70 100 μm), a much smaller dimension than can be achieved with human imaging techniques.
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These features are difficult to achieve with human samples because normal human cartilage is usually not available and cartilage from OA or RA patients normally shows drastic signs of matrix destruction/alteration, which often extend from the surface throughout the cartilage matrix.
To validate the expression of HBB in other human normal tissues, SQ-PCR was achieved with 16 human normal tissues.
Complete eradication of WT in multiple xenograft models was achieved with a human NCAM antibody drug conjugate.
sRANKL capture was achieved with recombinant human OPG R&D Systemss, Minneapolis, MN, Cat# 185-OS/CF) precoated onto a microplate, while sOPG capture was achieved by precoating microplates with soluble recombinant huRANKL (R&D Systems, Cat# 390-TN/CF).
This property of the rapid-acting insulin analogs offers the greatest advantage to patients in terms of practicality and flexibility that otherwise cannot be achieved with the human regular insulin formulation.
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