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Stable transfection in HEK293/EBNA cells was achieved using the same vectors and Fugene 6 method as previously described.
Separation was achieved using the same column as above.
Relative quantification was achieved using the ΔΔCt method.
Tree construction was achieved using the Neighbor-Joining method [ 79].
23 Cell counting was achieved using the unbiased-stereologic method.
Using the same method, we achieve P e 02 = P e 03 = Q E s 2 N 0 2 TW + 2 N 0 E s (13).
The fact that a relatively high number of morphological features were used for each pairwise classification (i.e., 53, 80, and 99) compared to previous studies using the same method but achieving better between-group discrimination reinforces this notion.
The data collected were compared to the clinical outcomes achieved for embryos at the same developmental stage, cryopreserved using the same method, but without prior microsuction.
The pregnancy was achieved through IVF treatment; afterwards Wise and Thompson attempted to have another child using the same method.
Ms. Power, using the same method, became Alice L. Metzinger.
Soon, abortion opponents began using the same method.
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