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Following assay and reagent optimization, a sub-pM (25 amol) limit of detection could be achieved in buffer and also in neat, undiluted serum, representing a 160-fold improvement over that achieved using convention detection with a fluorescence plate reader.
The reported LOD of 1 ng/mL was achieved in buffer.
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Good linearity with R 2 = 0.997 was achieved in acetate buffer system.
In the practical quantitative analysis with immunoassays, a LOD of 145 fM for antiBSA was achieved in both buffer and interference protein samples.
97% release of diacerein was achieved in phosphate buffer (pH 7.4) with a half-life of 48 min, following first order kinetics.
Best performance was achieved in phosphate buffer at pH 7, showing a sensitivity of 0.57 mA M−1 cm−2, a detection limit down to 60 μM, and a linear domain up to 100 mM H2O2.
The electrochemical pretreatment of PGE was achieved in phosphate buffer (pH 7) by the potential recycling between 1.5 2.0 V/SCE for 100 cycles, and at the scan rate of 100 mV s−1.
Briefly, antigen retrieval was achieved in citrate buffer, and then blocked, followed by incubation with rabbit monoclonal Ki67 antibody (Thermo Fisher Scientific Fremont, CA).
The separation of ATP, AdoMet, and AdoHcy is dependent on the pH of the mobile phase (data not shown), with the best separation achieved in phosphate buffer at pH 7.2.
The in-vitro release studies indicated that the maximum % of IBM release (98%%) was achieved in pH 7.4 buffer at 37 °C within 48 hrs.
The highest immobilization yield (72%) was achieved in a phosphate buffer of 0.04 mM at pH 7.2, irrespective of the temperature.
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CEO of Professional Science Editing for Scientists @ prosciediting.com