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Standardizing the operational methods and evaluation of the single activities and a joint practice can contribute to achieve the validation of the application model.
Further studies are still lacking to achieve the validation of oxidative stress-related biomarkers to implement strategies suitable to improve the prevention and reduction of cardiovascular risk.
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However although the reference genes identified in this study are stable under a wide variety of parameters, such as developmental, tissue and various stresses, it is essential that each study validate the stability of the selected reference gene(s) to achieve the systematic validation of reference genes that is required to compare different studies [ 2].
In order to achieve the method validation scope efficiently, experimental design presents a very useful tool.
The extractions were optimized on real non-spiked samples by means of experimental design and the optimal conditions were selected to achieve the method validation.
There is significant investment in high cost, high throughput technologies, trained laboratory work force, and laboratory informatics to achieve the level of validation required by CLIA or CAP to offer the test for patient-care.
In order to achieve the better assessment, the validation process was done trifold: for winter, transitional and summer season.
Overall, a PRO validation needs to achieve the standards of validity and reliability [ 2].
Following the designing procedure presented in Section 3.2 for and for different values of , the best ANN structure is selected in terms of the highest achieved with the validation set.
An ardent reader may note that the detection accuracy achieved for the validation set of Nugache and Zeus is higher than that of the test set composed of Storm and Waledac.
This was not fully achieved for the validation group (60 of 239 patients).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com