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cDNA was synthesized from the total RNA using a high-capacity cDNA Achieve kit (ABI).
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Synthesis of cDNA was achieved with Sprint RT Complete kit (Clontech) or iScript cDNA synthesis kit (Biorad).
Whereas the iScript™ Select cDNA Synthesis kit and the Transcriptor First Strand cDNA Synthesis kit achieved the lowest scores (Additional file 1: Table S3).
QuantiTect® Reverse Transcription, First Strand cDNA Synthesis kit for RT-PCR and the Transcriptor First Strand cDNA Synthesis kit achieved 15 successful reads at the 0.0032% level, attaining a score of 5 (15/18).
To achieve more sensing-sites, KIT-5 mesoporous-silica with higher surface-area is assessed, resulting in good sensitivity but too high −ΔH° that brings poor repeatability.
AffinityScript Multiple Temperature cDNA Synthesis kit achieved 13 successful reads at the 0.0032% level, attaining a score of 2 (13/18) and the iScript™ Select cDNA Synthesis kit recorded 5 successful reads at the 0.032% level and one successful read at the 0.0032% level (12/18), attaining the lowest score of 1 (Additional file 1: Table S1).
Oculus Rift is a brilliantly achieved piece of kit.
Finally, the kit achieved good matching and repetition results, and no cross-reactions occurred between enterotoxins during the procedure.
The designed kit achieved a correctness coefficient of 0.98 and detected enterotoxin A in 23% of the samples.
Detection was achieved using ECL kit (Santa Cruz, USA).
Quantitative PCR reaction was achieved using commercial kit containing SYBR Green (Eurogentec, Seraing, Belgium).
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