Current reprogramming methods rely on ad hoc arguments and brute-force trial and error experiments to identify the relevant genetic lever points in the network whose manipulation can achieve cell fate switching.
Indeed, because partial inhibition of the Wnt-PCP pathway only affects the expression of Pax6 in the optic vesicle and not in the surface ectoderm, we believe that the developing eye relies on the Wnt-PCP-driven cell movements in the optic vesicle to keep its epithelial status at this developmental stage, which is in turn crucial to achieve its fate.
In a number of vertebrates, there is a core transcriptional hierarchy, which can explain some of the molecular decisions that retinal cells must make to achieve particular fates.
The study predicted the lake had a 50-50 shot at achieving that fate by 2021, given current trends.
There is no telling what he might have achieved had fate not dealt him a series of such vicious blows after United paid £17m for his signature in the summer of 2007.
Loss of all alleles of GSK-3, as achieved in the DKO ES cell line, leads to a loss of secondary clonal spheres, perhaps because they do not achieve a neural fate, but instead maintain a pluripotent fate or switch to a more mesodermal fate (Fig. 4C).
Love, Browne's novel tell us, lifts us up to achieve our destiny, and escape our fate.
To investigate the potential of purified human pancreatic ductal cells to achieve an endocrine fate, we used an adenovirus-mediated transgenic system.
Thus, a changing external environment is neither essential, nor adequate, to achieve histogenetically appropriate fates (although it should be noted that environmental cues may nevertheless provide negative feedback to fine-tune the proportions of cells that acquire particular fates [ 13 15]).
It takes the most preposterous of coincidences, in the face of which he is rendered helplessly, almost religiously awestruck by the forces of fate, to achieve the play's inevitably happy ending.
