Sentence examples for achieve confluence from inspiring English sources

Immunofluorescent staining reveals the activation of focal adhesion and subsequent organization of F-actin from the attached cell surfaces, which suggests the TriG microspherical substrate is favorable to ADC adhesion and therefore capable of promoting HDF proliferation to achieve confluence by turning over three times within 10 days.

Both early- and late-passage cells achieve confluence and the cell density at the point of confluence appears roughly equivalent.

Briefly, cells were grown in keratinocyte serum-free medium (Life Technologies, Carlsbad, CA, USA) to achieve confluence, followed by DMEM/F12 (Sigma-Aldrich, St Louis, MO, USA) supplemented with 10% calf serum for 7 days to promote differentiation.

The cells were seeded at 2.5 × 10 cells/cm, which is a sufficiently high concentration to produce attachment to the substrate within 24 h that does not require further proliferation to achieve confluence.

Human smooth muscle cells adhered, grew and achieved confluence on the surface of the hydrogel fibers.

Each 8×104 cells were plated on 8-well plate coated by poly-L lysine (GIBCO, CA) after achieving confluence at day 4 in culture.

In contrast, more than 95% of non-growth arrested HDFs attached readily and achieved confluence within 5 7 days but then gradually died out within 8 14 days in G-FCS media.

Cells were cultured until the adherent fibroblast cells achieved confluence.

In this environment cells proliferated and achieved confluence on all surfaces after three days.

Cells for chemotactic assays were harvested when they had achieved confluence.

When ASCs achieved confluence the concentration was 1 × 10 ± 1× 10 EVs/ml that corresponded 1 × 10 ± 1× 10 per cell.