The phrase "achieve buffer" is not correct and does not convey a clear meaning in written English.
It may be intended to refer to reaching a certain level of buffer or reserve, but without context, it is unclear when to use it.
Example: "To ensure smooth operations, we need to achieve buffer before the peak season begins."
Alternatives: "reach a buffer" or "establish a buffer."
A series of Mes/HEPES pH buffers were previously prepared by mixing Mes buffer (50 mM Mes, 50 mM NaCl, 30 mM Ammonium Acetate, 40 mM Sodium Azide (Sigma), pH 4.0) with HEPES buffer (50 mM HEPES, 50 mM NaCl, 30 mM Ammonium Acetate, 40 mM Sodium Azide (Sigma), pH 7.5) to achieve buffers of varying pH, ranging from pH 4.0 to pH 7.4.
For Ca2+ titration, Ca2+/EDTA, Ca2+/HEDTA [ N- 2-hydroxyethyl)ethyleN- 2-hydroxyethyl N- 2-hydroxyethyl], and Ca2+/NTA buffethylenediamineed by mixing Ca2+-saturated aNd Ca2+-free buffers (30 mM Mops, 100 mM KCl aN′ 10 mM chelating reagent, pH 7.2, either with or without 10 mM Ca2+) to achieve buffer-free Ca2+ concentrations from 0 to 1.13 mM.
This counter-intuitive strategy of dispersion-enhanced chromatography refolding (DCR) is potentially generic for other proteins, and suggests the need for gel-filtration refolding columns and resins that achieve adequate buffer exchange yet maximise, rather than minimise, peak broadening.
Following assay and reagent optimization, a sub-pM (25 amol) limit of detection could be achieved in buffer and also in neat, undiluted serum, representing a 160-fold improvement over that achieved using convention detection with a fluorescence plate reader.
The fall-off assembly is inferred to achieve effective hydrodynamic buffer in drop accident during normal operation.
The policy expects the retrieval process of an object to finish right before the playback time of the object to achieve a better buffer utilization and network bandwidth efficiency.
Recall that states A and D correspond to oversensing and undersensing respectively while in states B and C, the thresholds are adapted to achieve a tight buffer region of stability.
A gradient of eluents A (distilled water with 2% (v/v) acetonitrile, 0.5% (v/v) formic acid) and B (80% acetonitrile in distilled water with 0.1% (v/v) formic acid) was used to achieve separation from 4% buffer B to 50% buffer B in 320 min (0.2 μL/min flow rate).
For pH stability, the concentrated supernatants were mixed to the same volume of citric acid-sodium citrate buffer to achieve different pH values lower than 6.0 and with Tris-HCl buffer for pH higher than 7.0.
