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The TGS technique combines High Resolution Gamma Spectrometry (HRGS) with Three-Dimensional (3-D) low spatial resolution transmission and emission image reconstruction techniques to achieve assay goals.
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This spatial confinement scheme simultaneously achieved assay volume reduction and signal amplification to overcome the limited sensitivity of LSPR biosensing for cytokine detection.
If harmonization of immune assays can be achieved, assays can be tested as surrogate endpoints in clinical trials, substantially accelerate the development of immunotherapeutic agents and, in addition, offer a rationale to pre-select groups of patients with high probability to benefit from subsequent immunotherapy.
Measuring tissue cocaine levels in de-chorionated zebrafish embryos was impractical due to the very large number of subjects per sample required to achieve the assay sensitivity threshold.
These results suggest that the reason why we are able to achieve high assay quality is largely due to the ability to examine a narrow range of AR expression that is essentially free from potential over-expression artifacts.
When we used LN215 or HOS cells, 10 s was enough to achieve excellent assay performance.
In an attempt to achieve an assay that can sensitively detect soluble oAβ in human biological fluids, we systematically evaluated several mAbs and two detection platforms.
Our objective was to achieve 100% assay completion rate, call rate and genotyping accuracy rate, for multiple SNPs across multiple samples.
To achieve an assay sensitivity comparable to manual analysis, we also defined the minimum number of individual larvae analyzed per condition to be 30.
Moreover, achieving acceptable assay sensitivity using DBS proved technically difficult in a less-experienced laboratory.
Perhaps the biggest obstacle to achieve acceptable HTS assay performance metrics occurs in 3D tumor models that produce spheroids with highly variable morphologies and/or sizes.
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