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Replicates of AIV A/Teal/Tunka/7/2010 (H3N8) were diluted in culture media without foetal calf serum to achieve a virus concentration of approximately 1 × 10 PFU in 500 μl.
MedImmune production process grows MDCK cells on Cytodex 3 microcarriers and serum-free medium to achieve a virus yield of 8.9 log10 FFU/mL, as measured by the fluorescent focus assay (FFA) [ 73], which is consistent with a high viral yield recovered from large-scale MDCK cell culture using roller bottles (1.0 · 10 PFU·mL−1) [ 74].
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The stability of EBOV in human blood was assessed by spiking blood samples from a healthy human volunteer to achieve a 10 TCID50/mL virus titer.
Our finding suggests that an efficient intranasal vaccination with a live attenuated H5N1 virus may require a certain level of pH and temperature stability of HA in order to achieve an optimal virus uptake by the nasal epithelial cells and induce a sufficient immune response.
Plaque purification was performed using each strain to achieve a highly homogenous virus clone, as described previously [ 17].
Testing GDA the concentration had to be increased up to 2500 ppm to achieve a 4 log10 reduction in virus titre.
However, it is difficult to achieve a complete removal of viruses by antiviral drugs targeted to the viral enzymes from patients persistently infected with RNA viruses that exhibit a quasispecies nature, such as human immunodeficiency virus (HIV) and HCV.
Together these findings suggest that it will be possible to achieve a fuller understanding of RNA virus evolution that integrates the molecular biology of the virus and the immune response of the host with the evolutionary changes.
A lower exposure time may be required to achieve a 6 log10 reduction in viable virus as Adenovirus is likely to become non-viable before DNA becomes non-detectable by PCR.
The concentration of virus required to achieve a 50% transfection rate was determined as TD 50 (Transfection Dose).
Following filtering, viruses are subsampled to achieve a more equitable temporal and geographic distribution.
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CEO of Professional Science Editing for Scientists @ prosciediting.com