The randomized sensitivities are achieved by constructing a library of synthetic gene circuits with randomized control sequences (e.g., ribosome-binding sites) in the front element.
Insert size selection was carried out using the Life E-gel SizeSelectoto achieve a mean library fragment size of approximately 600 bp for the MiSeq and 550 bp for the HiSeq2500.
To achieve these capabilities GeoPAT includes a library of scene signatures – compact numerical descriptors of patterns, and a library of distance functions – providing numerical means of assessing dissimilarity between scenes.
To achieve this goal, we curated a library of 329 amino acid sequences sampled broadly from across the CMGC group and then reconstructed their evolutionary history using maximum likelihood phylogenetic methods (Thornton, 2004; see 'Materials and methods').
This was achieved by the creation of a library of mutant pathways using de novo assembly of promoter mutants for each pathway gene in a target microorganism followed by high-throughput screening/selection [ 91].
One way to achieve such designs is to create a library of unit cells (the scaffold is assumed to be a repeating, tessellating unit structure), which can be assembled through specific computational tools proposed by several authors.
The final volume per well was adjusted to 100 µL to achieve a final drug library concentration of 50 µM in the drug screen.
High-throughput substitution is achieved by codon randomisation, which generates a library of mutants (a randomised gene library) in a single experiment.
The size of the library has evolved in multiple cycles to achieve a number of 400,000 compounds the time the experiments of this study were carried out.
The libraries were pooled to achieve a total diversity of 4.4 × 1011.
