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The final broth was incubated for 36 ± 3 hours to achieve a density of 1.6 2.0 × 108 cells ml-1.
During mammalian infection, B. hermsii produces cyclic spirochetemias that may achieve a density of 10 bacteria/ mL of blood.
As this culture reached an OD600 of 0.5, electrode bioreactor growth was initiated to achieve a density of 0.125 OD600 in the bioreactor.
Cells from frozen aliquots (-80°C) were thawed and added to achieve a density of ~108 bacteria/mL after 1 hr, at which time phage were added.
Briefly, an inoculum was prepared by suspending a 48-h culture in reduced Brucella broth (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA) to achieve a density of 1.0 on the McFarland nephelometer standard.
Our results also suggest that women at high risk of breast cancer may have to engage in more intense physical activity to achieve a density reduction compared with women at lower risk.
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A compromise between the different parameters and scan strategies was achieved and used to produce parts achieving a density of 99.8%.
First, bacteriophage were mixed with exponentially growing bacteria that had achieved a density of approximately 1 × 10 cells/mL.
When cells achieved a density of 5×10 cell/cm, cells were treated with 12 µM CHIR99021 (Selleckchem) in RPMI/B27 without insulin for 24 h (day 0 to day 1).
The pellet was re-suspended with medium to achieve a cell density of 2.105 cells/ml (cell density measured with a haemocytometer) and the obtained suspension was cultured in the dark at 26°C in an orbital shaker at 80 rpm.
"Canals are a brilliant idea in urban planning, because they allow you to achieve a higher density than you get in the suburbs and yet enjoy open space," said Barton Myers, a professor of architecture at the University of California at Los Angeles.
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