Yeast assays were done using conventional lithium acetate-based method.
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Animals were stained overnight with 0.5% uranyl acetate in veronal-acetate buffer (pH 6.0), dehydrated, and embedded in Spurr's resin.
The particle size of CHON-based NPs increased in PBS, acetate buffer and in HCl solution compared to that in water, but most of them remained in the nano-range even after 24 h.
The amplification products were separated on 2% agarose gel in Tris acetate EDTA buffer.
The hydrogen production increased along with acetate and buffer concentration.
Can HEPES be replaced by acetate as buffer?
(b) Can HEPES be replaced by acetate as buffer?
After that, they were washed twice with acetate buffer (0.1 M sodium acetate, 0.5 M NaCl, pH 4.0).
The resulting cRNA was fragmented at 94°C for 35 minutes in 40 mM Tris-acetate buffer, pH 8.1, 100 mM K-acetate and 30 mM Mg-acetate.
A baseline for the determination of metals in acetate buffer is comparatively low, suggesting acetate buffer as the best accumulating medium.
