Amount of H2S in zinc acetate traps was determined spectrophometrically at 667 nm after leaving the mixture for 15 min at room temperature.
The homogenate was mixed with 0.5 mL of 1 M HCl in a test tube to release H2S, and H2S was absorbed in a 1% (w/v) zinc acetate (0.5 mL) trap, located in the bottom of the test tube.
Endogenous esterases within the cell cleave the acetate groups, thus trapping the reduced form of the probe (DCHF) intracellularly.
An aliquot of plasma (100 μl) was added with 2% zinc acetate (200 μl) to trap the H2S, and 10% trichloroacetic acid (200 μl) was added to precipitate plasma proteins, immediately followed by 20 mM N, N-dimethyl-1,4-phenylenediamine sulfate in 7.2 M HCl (100 μl) and 30 mM FeCl3 in 1.2 M HCl (100 μl).
For example, one stage in the commercial manufacture of synthetic camphor involves a Wagner-Meerwein rearrangement of camphene to isobornyl acetate; here acetic acid acts both as a solvent and as a nucleophile to trap the rearranged carbocation.
In the presence of AuCl, ω-hydroxy propargylic acetates undergo a 1,3-acetoxy migration to form allenyl acetate 360, which is trapped intramolecularly to form tetrahydropyranes 361 containing an exocyclic enolacetate (Scheme 113).
TRAP staining was performed using TRAP buffer (50 mM sodium tartrate and 0.12 M sodium acetate, pH 5.2) with naphthol AS-MX phosphate and fast red violet LB salt (Sigma-Aldrich) as described previously [ 23].
In the blends with higher EVA concentrations, the effects of positron irradiation were appreciably weakened, as the result of positron trapping on the polar acetate group of EVA.
Within the cell, esterases cleave the acetate groups on DCFH-diacetate, thus trapping the reduced probe (DCFH) intracellularly.
Large aggregated AR complexes trapped by the cellulose acetate (CA) membrane were markedly reduced in the DHT-treated cells overexpressing GRP78/BiP, whereas levels of soluble monomeric AR protein trapped by the nitrocellulose (NC) membrane were only slightly reduced.
