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Following primary antibodies, sections were incubated with appropriate secondary antibodies conjugated with rhodamine (red) or aminomethylcoumarin acetate (blue).
Larvae are tested for their olfactory preference regarding (A ) n-amyl acetate (blue cloud), (B ) 1-octanol (gold cloud), or (C ) for their choice between n-amyl acetate and 1-octanol.
For example, in the left-most panel, a group of larvae is first (upper row) exposed to n-amyl acetate (blue cloud) together with fructose (green circle), and subsequently (middle row) to 1-octanol (gold cloud) without any tastant (white circle).
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Porcine liver esterase (PLE), digitonin, α-naphthyl acetate, fast blue RR salt, goat anti-rabbit HRP conjugate polyclonal antibody, and diisopropyl fluorophosphate (DFP) were purchased from Sigma Chemical Company (St . Louis MO).
The acetate-derived blue aromatic polyketide, actinorhodin, whose production is encoded by the extensively studied act genes, is absent from bldA mutants.
Paraquat (500 μM), lumiflavine-3-acetate, methylene blue, phenazine methosulfate and indigo disulfonate (2 μM each) were used as redox mediators.
Spectroelectrochemical experiments were performed using 650 μL samples containing 4 6 μM of wild-type or mutant NdCld in 150 mM phosphate buffer, pH 7.0, plus 100 mM NaCl, in the presence of various mediators: methyl viologen, lumiflavine-3-acetate, methylene blue, phenazine methosulfate, and indigo carmine.
Similar shapes, cut from red, yellow and blue acetate (suggesting three-color reproduction), are layered onto wire hangers.
After separation on silica gel using 45% ethyl acetate in hexane, blue spots were detected with Folin reagent.
After separation on silica gel using 45% ethyl acetate in hexane, blue spots were detected with Folin's reagent.
He introduces a segment where former bandleader Harold Defan reminisces about pianist Burnell Santiago as Mr. Defan listens to Santiago's only recording, a scratchy acetate of St . LouisBlues recorded in 1942.
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