Even some scientists associated with the public project say Celera has a more complete and accurate sequence.
Genomes usually must be decoded several times over to get a complete and accurate sequence, but the first three billion bases of Neanderthal should "hit all the essential differences," Dr. Egholm said.
And while the Government's Human Genome Project will soon have the whole kit and caboodle, Dr. Venter said Celera had a more accurate sequence and more powerful tools for analyzing the data.
With so much effort and scientific glory at stake, each team remains highly critical of the other's approach and believes its own has or will lead to the more accurate sequence.
The cDNA gives the accurate sequence and the mRNA indicates potentially modified bases.
The consortium says it's now ready to enter "phase two" and turn its collective sequencing firepower to finishing the human genome--that is, producing the 99.99% accurate sequence that has now been reached for chromosome 21 and 22.
However, post-mortem DNA decay rapidly reduces the number and quality of surviving DNA fragments, consequently increasing rates of sequencing error and forming a significant obstacle to accurate sequence reconstruction.
Our future study will focus on complex services, which are decomposed into plenty of atomic services, and make sure the atomic services of the complex service are executed in the accurate sequence.
After obtaining the PCR product with accurate sequence of the ORF2, we used TA cloning to transfer the PCR products using TA Cloning Kit with PCR 2.1 vector (Invitrogen, USA).
Further, recent developments in computational resources suggest that merging more accurate sequence and structure searches starting from differing sets of queries will not be limiting in the near future.
It was also concluded that the most accurate sequence for genome comparison purposes results from hybrid 454-Illumina sequencing coupled with the use of multiple bioinformatics tools for identifying differences between genome sequences from closely related strains.
