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This would suggest that the SNP frequencies are not very accurate below about 2-102-10%
SNP frequencies are not expected to be accurate below 1/N where N is the number of template molecules, and N appears to be about 10 to 50.
The Ct value was found to deviate from linear trend of the calculated standard curve when the amount of DNA used was less than 0.01 ng/μl (10 pg/μl), suggesting that the quantification was not accurate below this concentration [ 22].
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The location of our peak difference for the M170 was within 13 mm of that reported in an intracranial study (Allison et al., 1999), suggesting that the beamfomer localization was reasonably accurate (although see below: Comparison with previous studies – M170).
However, Button's reported salary at McLaren of £6million a season over three years is, if accurate, far below what has been commanded by other world champions in recent seasons, and Jordan believes there was never a deal on the table for Button to drive for the rebranded Mercedes Grand Prix team.
It has been shown that consideration of velocity overshoot is important for accurate prediction below 40 nm.
The service, which is available to Australians only, is being called the first legal download service that allows DVD burning (which isn't accurate – see below).
Even more accurate positioning (below 0.1 μm) can be achieved in the "only bending" mode by simultaneous bending of all legs in a desired direction and without executing the legs' stretching.
Using the same primer and probe set, Asahi-Ozaki et al.[ 6] displayed a linear relationship from 10 to 10 copies for both the HHV-8 ORF73 and GAPDH assay, but close examination of their HHV-8 ORF73 amplification curves shows tight replicates down to 10 copies: It is unlikely that the assay can yield accurate results below this level with any degree of accuracy.
For the lesser precision ((eta= 2^{-3}), bottom row in the figure), the accurate fractions decrease to below 0.2.
Update: We didn't see any reason to doubt the slides as legit, but we've gone ahead and confirmed with a few sources that at least some the details below are accurate.
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