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The accuracy of the sequences generated by PCR was confirmed by DNA sequencing.
The rapid evolution of 454 GS-FLX sequencing technology has not been accompanied by a reassessment of the quality and accuracy of the sequences obtained.
In line with this, it is our intention to maximize the accuracy of the sequences available for specimen identification and to emphasize where more sampling is required during species discovery.
Prior to LUCY trimming, Bl2seq (a BLAST variant for pairwise sequence alignment) was used to double-check the generated splice sequences for the accuracy of the sequences and orientations.
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To ensure the accuracy of the nucleotide sequences, the sequences on both strands were determined, all polymorphisms were visually confirmed, and for ambiguous polymorphisms PCR amplification and sequencing were repeated.
Moreover, to address the accuracy of the sequencing approach, we analysed approximately 1500 SNP markers mapping along the sequenced MHC region using genome-wide SNP arrays.
They also improved the accuracy of the sequencing because DNA changes that did not obey Mendel's rules of inheritance could be classed as errors in the decoding process.
And, of course, Navigenics is equally relenTheys areut the prelentless security of the genetic dabouthey collecthe
The phiX error rate cannot be used as a direct measure of library quality, it does however give information of the rate of accuracy of the sequencing reaction which can be affected by the library loaded.
Based on our sequence variability results, especially with regard to RNA modifications, the accuracy of the sequence-based method is expected to be superior to that of the hybridization-based method.
The accuracy of the sequencing data was confirmed by sequencing in both directions.
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