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However there are no truth standards so our results reflect the precision but not necessarily the accuracy of the platform.
To account for the lower breakpoint accuracy of the platform used in our study, we extended the parameter sensitivity analysis to include flanking regions ranging within 100 2,000 bp.
These results validate the quantitative accuracy of the platform when using multi-spectral time-gated data sets.
The authors used a relative error calculation to describe the dependence of the accuracy of the platform on the number of probe pairs per transcript.
The Illumina miRNA profiling system has been described previously, showing the accuracy of the platform by rt-PCR and digital sequencing [ 21] using RNA from four cell lines (the same cell lines were used in this report) and purchased human tissues from Ambion.
A list showing all of the genes differentially regulated for each experiment (FDR = 0) is provided in Additional files 1, 2 and 3 To validate the microarray data and evaluate the accuracy of the platform, we performed quantitative real-time PCR (qRT-PCR) on 12 genes.
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This paper proposes a new mixed beam-shell model for the seismic analysis of reinforced concrete coupled walls with sufficient efficiency and accuracy on the platform of general finite element software MSC.Marc.
In addition, the required accuracy on the platform position is analyzed at the end of the paper.
Within-platform correlations provide an estimate of the array reproducibility, while comparison to RT-PCR provides insight into the accuracy of the platforms as described in Irizarry et al. [ 18].
The estimation of functional parameters was investigated by multispectral time-resolved spectroscopy and the composition of the mixture was estimated accurately with an error less than 6% demonstrating the quantitative accuracy of this platform for functional imaging studies.
However, the variance of the recovered fold-changes was significantly larger for the TaqMan platform than the miRCURY platform (p < 0.001, variance comparison test)) indicating a poorer recovery accuracy of the TaqMan platform.
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