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In our current accumulation protocol, the normal accumulation of open wild-type KCNQ1/KCNE1 channels occurs during increased depolarization frequency without adjusting the duration of the depolarization.
For the K+ conductance accumulation protocol analysis, outward current during the first 10 ms of the beginning of each depolarization was averaged.
To confirm that the effects of HCQ on rate reduction were likely to arise from the modulation of If without the significant contribution of other mechanisms, we performed a sequential accumulation protocol similar to those performed by Verrier et al. Near-maximal inhibition of If was achieved using 1 µM ZD7288 (If inhibitor), followed by cumulative doses of HCQ (Supplementary Figure 1).
As with all proposed SBAs, HCQ will exert effects on pacemaking mechanisms beyond modulation of If as dose is increased; the results of our sequential accumulation protocol with ZD7288 are promising, suggesting that HCQ does not reduce beating rate under conditions in which complete block of If has already been achieved.
Similar(56)
On the other hand, 15N-labeled N2 accumulation using the same protocols was hardly detectable in the northwestern Bering Shelf water during the same cruise, even though it is considered to have a similar ecosystem as the Chukchi Shelf [11, 13, 48].
We found that under 6T15 protocol no significant accumulation of G2/M cells was observed (Fig. 3B) suggesting that the failed accumulation of G2/M cells was responsible for the lack of miR-290 up regulation.
Citrate is first-choice anticoagulant in CRRT, but invest in understanding, stick to the protocol and monitor accumulation.
The repeated milking protocol (10-min accumulation) did not significantly affect the proportion of RNA attributable to crescents or nucleated cells.
For example, in a 50-node network SNCF caused accumulation of around 250 frames while CA protocol resulted in a number less than half of that.
The cells were lysed, and cAMP accumulation was measured according to the CatchPoint protocol.
The EtBr accumulation assay was performed following a published protocol.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com