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Although the two-layer cascade CHA reaction generates greater amplification, non-specific hybridization among the numerous CHA substrates can again lead to the accumulation of background.
However, it was also clear that other mechanisms besides disomy at chr 13, such as the accumulation of background mutations, may result in reduced melanin production.
While enzymatic probes are capable of amplifying the signal, resolution can be compromised due to the diffusion of the reaction product and accumulation of background.
PALMIRA also reduces background noise to a minimum: While frame rates faster than 1/ ton spread bursts over several frames, increasing readout noise, slower frame rates would result in additional accumulation of background.
This may be important if repeated pH cycling is required, as the otherwise problematic accumulation of background salt is minimized.[ 11] It is also shown that a pH switch can induce an irreversible vesicle-to-worm morphological transition.
However, due to the accumulation of background mutations during the mutagenesis and/or evolutionary engineering procedures used to develop the strain, GS1.11-26 did not retain the same tolerance to high concentrations of ethanol and acetate, and showed reduced ethanol accumulation capacity in very high-density fermentations compared to the original Ethanol Red strain.
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To minimize the accumulation of the background fluorescence that is generated by leaky, undegraded level (i.e. from previously expressed fluorescent protein that has not yet degraded), several chimeric EGFPs with shorter fluorescence half-lives were constructed by fusing protein [ 71, 72].
A high CFD total score, representing an accumulation of unfavourable psychosocial background factors (e.g. low family income, poor social network and parental psychopathology), significantly predicted future criminality (p < 0.01).
This may represent the accumulation of the genetic background (e.g. HLA class II susceptibility alleles (DRB1*1501), IL2R or IL7R for MS) [ 48], in conjunction with the shaping of the immune repertoire during ontogeny and the presence of stochastic infectious challenges for generating individuals susceptible to develop autoimmune diseases.
The smaller effective pore size at the open-channel/gel interface presents an issue for probing, particularly for higher %T gels, because it is this accumulation of high antibody background at the interface that obscures the probe signal from the antigen, greatly reducing assay sensitivity.
Because the referent serum concentrations were assumed to be the accumulation of a lifetime of background environmental exposures, we assigned the TCDD serum values from the examination to each referent pregnancy.
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