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Following extended treatment with PIs, and concomitant with PreA accumulation, cells acquire irregular nuclear profiles with quantifiably decreased circularity (Figure 2A C).
For accumulation, cells were treated with 10 mM 2-DG before incubation with [H]nifurtimox as described above.
To investigate effects of mutants expressing efflux pumps on cell growth and alkane accumulation, cells harboring a recombinant plasmid were grown in an induction medium plus alkanes.
To determine whether the increase of LC3-II with FAC was due to autophagy induction or autophagosomal accumulation, cells were cotreated with CQ and FAC.
Following Rh123 accumulation, cells were washed twice with ice-cold Hanks' Balanced Salt (HBSS) (without phenol red), placed in HBSS with 10% fetal bovine serum on ice.
For mitoxantrone (MX) accumulation cells were incubated in the presence of 5 μM MX (Sigma) in the presence or absence of fumitremorgin C (FTC; 10 μM; Sigma).
Similar(47)
Similar to ROS accumulation, cell membrane permeability trended with FFA production (7942 < SE01 < SE02).
These results show that the enzymatic inhibition of Nox4 prevents oncogenic Ras-induced ROS accumulation, cell hyperproliferation and DDR activation.
Of special interest were genes annotated to be involved in anthocyanin accumulation, cell growth, flower development and other developmental processes.
This model takes into account cell mortality, non-viable cell accumulation and cell recycling.
(F ) Timecourse of accumulation of cells with acetylated tubulin during muscle cell differentiation.
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