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Although the bioenergetic gene repertoire varies among the several eukaryote domains (plants, fungi, protists, animals), two important ideas emerge from the accumulating sequence data.
The exponentially accumulating sequence data in GenBank have allowed for the publication of hundreds of nucleotide substitution rate estimates for the rapidly evolving RNA viruses.
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Previous results have shown that an automated clustering alone does not join all possible overlaps due to accumulating sequencing errors in the end region of the sequences.
Typically it appears that when accumulating sequenced clones in a positive-selection screening approach, mutations will be enriched in a limited number of genes.
Thanks to the technological advances that began with the development of DNA sequencing methods in the mid-1970s, DNA sequencing is becoming faster and cheaper, but, disturbingly, the accumulating sequences are greatly exceeding our ability to interpret the final products.
In the formation of a quasispecies, the population minimizes the mutational load by accumulating sequences that have a reduced probability to suffer from deleterious mutations [ 27, 28, 39, 40].
Do these features relate to robustness — the ability to readily accumulate sequence changes?
Although the Nipponbare RefSeq is high quality, the rice seeds would accumulate sequence variation during propagation each year.
Based on accumulated sequence data from the Los Alamos HIV Database (HIVDB) for HIV-1 subtype B Nef, we synthesized peptides corresponding to the consensus sequence and several naturally occurring mutants.
Thus, rather than directly assessing the degree of conservation among sequences, phylogenetic shadowing reveals regions that have accumulated sequence variation at a relatively slower rate, indicative of conserved function [46].
The minimum allele quality (accumulated sequence quality for every allele) was not lower than 20.
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