We absolutely agree that different types of mutations would accumulate differently, and have expanded the text to describe this issue and justify our modeling of a particular class of mutation those that directly affect intracellular replication rates.
Numerous studies have investigated the expression of the RBCS genes in response to light or in different tissue types and have shown that transcripts from individual genes accumulate differently [for a review, see [ 63]].
We suggest that this cost may accumulate differently throughout the development of a trait to incur increased costs for plastic development.
This is in agreement with the fact that grasses display a marked developmental preference for accumulating differently modified pectins in specific cell types (Carpita, 1996).
As the two compounds may accumulate differently in the larvae, we examined levels of TBT and DBT in larval extracts after treatment with 80 nM of either DBT or TBT by HPLC MS/MS.
DOI: http://dx.doi.org/10.7554/eLife.02555.005 Since an end point of endocytosis is degradation in lysosomes, we further asked if wildtype Ptch1 and 'PPXY' motif mutants accumulate differently in the presence of proteasomal or lysosomal blocker.
However, in this study, we report a surprising finding that in vivo HGNS are unstable, fragmenting with the Au and the remnants of the sacrificial Ag core accumulating differently in various organs.
Such models can be traced back to 1735, when Reaumur proposed that the bioclimatic events of organisms and the dates of occurrence differ with regions, species, and altitude because the temperature required for each organism to grow and develop varies and accumulates differently according to region.
However, different defense-related proteins and metabolic enzymes were found to be differently accumulated during lesion formation in a lmm-specific manner or in different lmms.
The different categories of polyphenolic compounds were differently accumulated in the samples defined in the experimental design planned here.
