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Due to the constraints of a symbiotic lifestyle, it is expected that genes will accumulate slightly deleterious substitutions faster in symbiotic bacteria than in their free-living relatives.
On the contrary, we confirmed that, in berries, VvSUC11 and VvSUC12 transcripts are present at all developmental stages and accumulate slightly at the onset of ripening [ 30, 31].
Here, we will argue that their constructive phase can be explained by a more general property of mitochondrial genomes, namely the relatively high rate at which they accumulate (slightly) deleterious mutations (Neiman and Taylor 2009).
As shown in Fig. 3, white matter regions including the cortex and cerebellum seemed to accumulate slightly more radiotracer (leading to more baseline and background activity in the tissues).
KB/VCR cells were also observed to accumulate slightly more tanshinone-1 than the parental cells (Supplementary Figure S2b), and tanshinone-1 did not change the expression of the mdr-1 and MRP1 genes at either protein or mRNA (Supplementary Figure S2c) levels.
Both proteins appeared to accumulate slightly in endogenous VAPB immunoprecipitates upon proteasome inhibition, as determined by SILAC mass spectrometry (Table 5, Additional file 4: Table S3) and were identified at low levels in Flag-FAF1 immunoprecipitates (Table 6, Additional file 5: Table S4).
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Following multiple CMS doses, colistin reached steady-state within 24 h; there was no accumulation of CMS, but colistin accumulated slightly (RAUC = 1.33).
The accumulation persisted for at least 20 min (Fig. 6A,B) and although the ezrin-EGFP accumulated slightly within this time frame, accumulation using pilus-coated beads was significantly higher at this time point (Fig. 6B).
In addition, businesses, excluding auto dealerships, accumulated slightly more inventory in December than earlier thought.
In its climate it is more remote, less dynamic, less subject to ocean influence, and as a result the area accumulates slightly more heat than Napa.
Both 2KO lines accumulated slightly less transferrin, and for the RabX2 knockout this was ∼20% less than wild type.
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