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A 10 bp overlap between the partner sequences was allowed at the fusion point to account for alignment uncertainties.
In the next section, we extend these ideas to also account for alignment sparsity.
As reported in table 2, only for ALB both null models were rejected in favor of the positive selection models (after Bonferroni correction for two tests, to account for alignment splitting).
Sufficient read depth was available to survey ASE in nine of the participants (see "Methods" for description of cutoffs and procedures to account for alignment biases [ 41]; we report here alignments to each individual's own genome as these are closer to the expected transcriptome-wide absence of bias).
An alignment of the CM to a particular sequence is represented as an SCFG parse tree, a state path through the consensus guide tree, using match, insert and delete states to account for alignment positions, and start, bifurcation and end states to account for the branching tree structure itself.
The total energy of the charged defective systems were corrected[ 26] to account for: alignment of the electrostatic potential between the bulk and the defective supercells; finite size effects in the calculation of charged impurities; band filling by defect levels resonant in the band edges.
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We optimized MP overspotting pin diameter, accounting for alignment error, to allow construction of large, high-fidelity arrays.
Thus, even accounting for alignment uncertainty, we achieve an increase in the phylogenetic resolution.
However, recent work suggests that the relationship between BML and progression persisted after accounting for alignment [ 18].
Accounting for alignment bias and uncertainty would be an important consideration for testing homology hypotheses in cases of very little sequence similarity.
Reads were assigned to genes in a hierarchical fashion beginning with reads with only a single match, followed by reads with two matches, etc. Accounting for alignment ambiguity was important for TEs because they are repetitive by nature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com