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Skeletal muscle biopsies were collected accordingly to previously published procedures (Cooke et al. 2011).
Cells were treated with recombinant human IL-10 (R & D Systems) at various doses (50, 100, or 500 U/ml) and for different times (at 6-hr intervals during a 72-hr culture period) accordingly to previously reported conditions [ 7].
Using the EAdi waveform, we quantified the three main types of asynchronies accordingly to previously published definitions [ 7, 25] (see also Additional file 2): (1) ineffective efforts; (2) auto-triggering, and (3) double triggering.
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ICAM-1, VCAM-1 and NF-κB were visualized in HUVEC by indirect immunofluorescence, accordingly to a previously described protocol [32].
Development and validation of analytical method by HPLC-IR for evaluation of artemisinin in A. annua L. was accordingly to method previously developed and validated by our research group [ 10].
The zone is rotated in the opposite direction accordingly to the technique previously presented.
Western blotting was performed on cytoplasmic cell lysates to detect MOS and β-actin proteins accordingly to the methods as previously described [ 33].
Reactions were run at similar conditions as previously stated with 125 μM tyramine substrate for MAO A and with 125 1,000 μM tyramine substrate for MAO B. Progress curves were then fit accordingly to eqs 1– 3 as previously stated.
The functional assignment for each enzyme sequence family, i.e. HisA, PriA and subHisA, was done accordingly to the amino acid residues previously identified as specific for each sub-family, as describe in the Background section and references therein (see also Additional file 1: Figure S1).
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