The former responsible to infer user control command while the latter controlling the robot arm movement according to the user instruction.
Color was developed and absorbance reading was performed in ThermoMax microplate reader (Molecular Devices, Sunnyvale, CA) at 450 nm and subsequent analysis was performed according to the user instruction of Methylamp Global DNA Methylation Quantification Ultra kit.
After nitroglycerine 0.2 mg and fentanyl 0.02 mg intravenously, catheter ablations were performed in a helical pattern with the goal of at least 4 6 ablations per renal artery to cover each short axis transaxial quadrant, according to the user's instruction of the device.
RNA was isolated using RNeasy Mini kits (Qiagen, Hilden, Germany) according to the user's instructions.
Sequencing of the IMAGE cDNA clones, PCR and RT-PCR products were performed using the dye terminator chemistry (Big Dye Terminator Cycle Sequencing II kit, Applied Biosystem, Foster City, CA, USA) according to the user manual instructions and analysed using an automated sequencer (ABI 3100; Applied Biosystem, Foster City, CA).
Each cRNA (750 ng) was hybridized to RatRef-12 Expression BeadChip (Illumina, San Diego, CA, USA) containing 22,523 probes for a total of 21,910 rat genes selected primarily from the NCBI RefSeq database (Release 16) according to instruction provided by Experienced User Card (11286340 Rev A, Illumina).
The API ZYM strip was inoculated according to the instructions in the user manual for the bioMérieux API ZYM set and was incubated for 4 h at 37°C.
Samples were inoculated according to the instructions in the user manual for the bioMérieux API 20E set and were incubated at 36°C for 22 h.
Respectively 5 × 10 and 1 × 10 MUG-Myx1 cells were seeded in electronic microtiter plates (E-Plate™; Roche Diagnostic) and measured for 92 h with the xCELLigence system according to the instructions in the user's manual.
