Sentence examples for according to the protocols recommended by from inspiring English sources

The extracted RNA was treated with RNase-free DNaseI (Frementas) to eliminate genomic DNA contamination according to the protocols recommended by the manufacturer.

Cell free supernatant was collected from T cell-DC co-culture system 72 h later and levels of IFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2, and IL-17 were measured by CBA assay with the Human Th1/Th2/Th17 Kit (BD Bioscience) according to the protocols recommended by the manufacturer.

Cell free supernatant was collected 24 h later and levels of IL-12p70, TNF-α, IL-10, IL-6, IL-1β, and IL-8 were measured by Cytometric Bead Array (CBA) assay with the Human Inflammation Kit (BD Bioscience) according to the protocols recommended by the manufacturer.

The human prostate cancer cell lines DU145, PC3, LNCaP, CWR22 and CWR22-Rv1 were obtained from American Type Cell Culture Collection (ATCC, Rockville, MD, USA) and cultured according to the protocols recommended by the ATCC.

All reactions were carried out according to the protocols recommended by the manufacturer.

All the reactions were set up according to the protocols recommended by the suppliers.

ChIP assay was performed according to the protocol recommended by Upstate (Lake Placid, NY, USA).

The procedure was performed using a kit purchased from Upstate Biotechnology, according to the protocol recommended by the manufacturer.

RNA was isolated with an RNeasy Mini Kit (QIAGEN), according to the protocol recommended by the manufacturer.

The standard 5-µl polymerase chain reaction (PCR) reaction was carried out using TaqMan® Universal PCR Master Mix reagent kits according to the protocol recommended by the manufacturer.

The purified p66 proteins were subjected to NativePage Novex Bis-Tris gel analysis and Western blotting according to the protocol recommended by Invitrogen, USA.