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Affymetrix GeneChip Human Gene 1.0ST Array was used for gene expression analysis according to the protocols from the manufacturer.
The growth inhibition of various cell lines was determined according to the protocols from the NCI/NIH Developmental Therapeutics Program, and in collaboration with the Northwestern University Developmental Therapeutics Core Facility.
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Twenty nanograms of DNA template were used for WGA according to the protocol from manufactures.
Bioactivity data were extracted from ChEMBL16 [22] according to the protocol described by Koutsoukas et al. [10].
Nuclear extracts were prepared from lungs according to the protocol of Dignam et al. [16].
The proteins were purified from bacteria according to the protocol described earlier [29].
Informed written consent was obtained from all patients according to the protocol approved by the Louisiana State University Health Sciences Center LSUHSC Institutional Review Boardrd.
These 28 MRSA strains were removed from the analysis, according to the protocol.
Total RNA was extracted from HT29 cells using easy blue (Trizole) according to the protocol.
injection, according to the protocol described below.
DNA was isolated according to the standard protocol from EDTA blood as previously described [ 24].
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