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The DRSC assay was conducted according to the previous method [9].
The lethal toxicity of the as-prepared BoNTs was determined according to the previous method [28].
Natural abrin was extracted according to the previous method with slight modifications [16].
And then the relative recovery (%RR) was calculated according to the previous method (Rezaee et al. 2006).
The mice were immunized with rOmpF (20 mice) or PBS (control, 20 mice) according to the previous method reported by Reddy et al. (2010).
DCFH-DA (2-dichlorodihydrofluorescein diacetate; Sigma) was used as a ROS-capturing reagent according to the previous method (Cai et al., 2008).
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RNA hybridization and detection were performed according to the previous methods (Sambrook et al. 2001).
The enzyme activities of various immobilized PAs were determined according to the previous methods (Ferreira et al. 2007; Shewale et al. 1987), with some modifications.
The rOmpF protein was purified by Ni2+-NTA affinity chromatography and refolded according to the previous methods (Guan et al. 2015; Saleem et al. 2012).
The purification of BoNT/B was completed according to the previous methods published by our laboratory without polyethylene glycol (PEG) precipitation and Sephacryl-200 column process [27].
All proteins were expressed and purified according to the previous methods used in D. melanogaster Ddc recombinant production [28] with modifications.
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