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Various other growth parameters such as maximum specific growth rate and doubling time (Td) were determined according to the method provided by Painter and Marr [24] and Levasseur et al. [25].
according to the method provided by the manufacturer.
The data of HR, V and O-E then could be obtained according to the method provided by Tierney JF et al. [ 10].
The mixed model method was used to analyze the repeated ear swelling measurements over time according to the method provided by Wang and Goonewardene [ 62].
p70S6K and MigR1 plasmids were from Addgene (Cambridge, MA, USA) and shRNA P70S6K were from Thermo Scientific/Dharmacon (Lafayette, CO, USA) and were transfected to the cells according to the method provided by the companies.
4.8) software, setting an exposure time of 900 ms to subtract the background autofluorescence signal of the A. sojae wt.> SSF experiments were carried out independently, in triplicates, and for 10 days with an untransformed (A. sojae wt) and a selected transformed fungal strain (A. sojae vgb+) according to the method provided by Heerd et al. (2014b) with slight modifications.
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The production of PI3P in each sample was detected using ELISA, according to the methods provided by the manufacturer.
For continuous variables, if the study provided medians and ranges instead of means and standard deviations, we calculated the means and standard deviations according to the methods provided by Hozo et al. [ 15].
Total protein assay was performed using the BCA protein assay kit, according to the Bradford method provided by the manufacturer.
The site-specific mutants of pRL-VEGF-Con1 and pRL-VEGF-Con2 were also established by using Takara MutanBEST Kit (Takara Corp., Dalian, PRC) according the method provided by the manual.
This study used a microplate fluometric assay modified according to the method proposed by Schwager et al. [21] to provide rapid screening and to consume fewer reagents.
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