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In the MAC module, the error control process is performed according to the instructions from the multiplexing module.
A ruling published in the Official State Bulletin stated: According to the instructions from the Ministry of Justice (Dirección General de Registros y Notariado), Spanish Consulates abroad may carry out the preliminary paperwork for a same-sex marriage.
SDS-PAGE and immunoblotting were performed according to the instructions from the manufacturer (BioRad, Hercules, CA).
Blots were developed with enhanced chemiluminescence using the appropriate horseradish peroxidase-labeled secondary antibody, according to the instructions from the manufacturer (GE Healthcare).
Protein carbonylation was then determined in the whole muscle lysate (20 µg protein) by an immunblot-based assay according to the instructions from the manufacturer.
Biopsies were then gently washed in PBS and fixed in 4% paraformaldehyde (PFA) or homogenized for RNA extraction using RNeasy Micro Kit (Qiagen) according to the instructions from the manufacturer.
All of the measurements and transformations above were calculated according to the instructions from the original technical paper [35], but the copy number used in the following case-control association analysis was the total copy number integer from the hidden Markov state in the original technical paper [35].
Essentially, the assay mixture contained 25 µl of cell extract and 100 µl of ATP assay mix (FL-AAM), which had been diluted 250-fold in adenosine 5'triphosphate (ATP) assay mix dilution buffer (FL-AAB) solution according to the instructions from the suppliers.
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Hybridization, staining, and scanning were performed according to the instructions from Affymextrix.
These algal cells were grown according to the instructions from CCMP and NIES.
FLIVO caspase staining kits were from Immunochemistry Technologies and used according to the instructions.
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