The microarray results were normalized to internal Affymetrix controls using GeneChip Operating Software GCOSS) and with a standard set of R methods according to standardized protocols (Affymetrix).
Both methods were given according to standardized protocols.
Six affected Greyhound show dogs underwent a complete neurological examination according to standardized protocols by different veterinary neurologists because of weakness and progressive gait abnormalities of insidious onset between three to nine months within the first year of life.
Analysis of bone volume, trabecular number, trabecular spacing, trabecular thickness, and the determination of osteoblast and osteoclast numbers and surface were carried out according to standardized protocols using the Osteo-Measure histomorphometry system (Osteometrics, Atlanta, GA, USA) [37].
Anthropometric measurements (height, weight) were conducted according to standardized protocols.
Height and weight were measured according to standardized protocols.
Samples were collected according to standardized protocols [ 36].
The mammary glands were cleared according to standardized protocols [ 30, 31].
Both ventilation and weaning from the ventilator were performed according to standardized protocols.
Trained study personnel performed anthropometric measures at the baseline visit according to standardized protocols.
Molecular screening was performed on genomic DNA extracted from frozen foetal tissue, according to standardized protocols.
