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> In a long-term prospective study in 6,350 healthy infants treated according to specified guidelines, Paradise et al. [ 38] observed bilateral OME in only 5.6% of the 161 children who had not received tympanostomy tubes at the age of 4. The indication to insert tubes is equivocal.
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All procedures involving the use of animals were conducted according to guidelines specified for the animal protection and welfare by the Spanish Ministry of Agriculture.
The design of this study is according to the guidelines specified by the Task force on promotion and dissemination (1995) and approved by the independent Medical Ethics Committee (METC) of the Utrecht Medical Centre at Utrecht University.
Luciferase reporter assay was performed using Luciferase Assay System (Promega) according to the manufacturer's specified guidelines.
miRNA was reverse transcribed using the Ncode miRNA first-strand cDNA synthesis kit (Invitrogen), according to the manufacturer's specified guidelines.
A putative miR-302d-recognition element (as single copy) from the CDKN1A or CCL5 gene was cloned in the 3'- UTR of the firefly luciferase reporter vector according to the manufacturer's specified guidelines.
The dose was specified according to the guidelines of the International Commission on Radiation Units [ 21].
Placental swabs for microbiology were obtained aseptically by histopathology staff from the chorioamnionic membrane interface from 30 cases and 85 subcohort, and cultured for aerobic and anaerobic bacteria, yeasts and genital mycoplasmas, according to hospital guidelines which specified that placentas from gestations less than 34 weeks should be submitted for pathological examination [ 13, 14].
This patient was replaced according to protocol specified guidance.
Data were simulated according to the specified probability models: a.
Active surveillance should include at least one re-biopsy according to UK guidelines that do not specify the timing (NICE, 2008); other guidelines recommend it within 18 months, then periodically (CCNS, 2006; NCCN, 2007) or every 3 years (BCCA, 2001).
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