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The cell stiffness was qualitatively analyzed according to reported methods [7, 8, 15].
The preparation of SUVs was performed according to reported methods.
According to reported methods [ 8, 9], 10 of 20 rats were randomly selected to establish the GIOP models.
Induction of LO product formation in isolated human neutrophils or monocytes, and analysis of the LO products, was performed according to reported methods.
Starting β-keto esters were either purchased from commercial suppliers or prepared according to reported methods.[ 25, 29 ] A detailed experimental section including all the procedures and analytical data of catalysts and fluorination products as well as copies of NMR spectra and HPLC chromatograms can be found in the Supporting Information.
The pH was monitored using a Denver Instrument UltraBasic Benchtop pH meter (Bohemia, NY), and solvents were filtered using 0.2 μm filters (Whatman, GE Healthcare or Durapore, Merck Milliproe) prior to use.68Ga was eluted from a 50 mCi 68Ge/68Ga generator (iThemba LABS, South Africa) and purified according to reported methods (Lin et al. 2015).
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The extracting procedure was modified slightly according to our reported methods [8] and described as follows.
RE- oleate 3 complexes (RE- oleate 3 Er) were synthesized acomplexesto pREviously reported methods [15, 27].
The anticonvulsant activity was measured according to the reported methods [42, 43].
Amino-functionalized gelatin/silica nanovectors (GS NPs) were first prepared according to previously reported methods [20].
3- p-tolyl)allyl-Neu5Ac2en was produced according to the reported methods.
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