Sentence examples for according to provided protocols from inspiring English sources
The RNA was then solublized and cleaned using DNase I and the RNeasy Mini Kit (QIAGEN Inc, Valencia, CA) according to provided protocols.
Cryopreserved cells were thawed and maintained according to provided protocols.
After thawing, samples were treated and analyzed according to provided protocols for the ATP Determination Kit (Invitrogen).
(VECs) were purchased from MatTek Corp., (Ashland, MA USA) and maintained according to provided protocol.
RNA from EMB samples was extracted using RNeasy fibrous tissue minikit according to provided protocol (Qiagen).
Oxidized and reduced glutathione were measured using GSH/GSSG-Glo assay provided by Promega according to provided protocol.
A 5 μg aliquot of tissue RNA, isolated as previous described [ 37], was used for reverse transcription carried out using random hexanucleotide primers and SUPERSCRIPT III (Gibco BRL) in a 20 μl reaction according to provided protocol.
The pyrosequencing was performed according to supplier provided protocols, and as previously described in [ 16].
Cluster formation, primer hybridization, and pair-end sequencing were performed according to the provided protocols [ 25].
DNA was harvested using NucleoSpin Tissue (Machery-Nagel. Düren, Germany) according to the provided protocols.
The luciferase activity of the p21Waf1 promoter reporter was determined using the Dual-Luciferase Reporter accordingtom (Promega) according thethe provided protocol, and normalized to the corresponding Renilla luciferase reporter activity.
