snf2Δ and mad2Δ mutants were constructed according to a PCR-based protocol (Longtine et al, 1998).
Subsequent steps of DNA extractions were performed according to the column-based protocol.
DPPIV− recipient rats were treated according to the RS-based protocol developed in our laboratories (Laconi and Laconi 2002).
According to its program description in the CHMI database, Viva Sehat provides doctor consultation and diagnostic services according to software-based standardized treatment protocols.
Patients were treated with various antidepressants and co-medication according to the protocol based on evidence-based clinical guidelines.
P19 EC cells were differentiated according to two RA-based differentiation protocols (Fig. 1a, schematic).
The iPlay programme was developed according to the intervention mapping protocol [ 1].
BSA mapping was performed according to the protocols of this paper (ATSNPTILE) or the protocols of Borevitz et al [3] (using previosuly obtained hybridizations of Col-0 and Ler-0.
Array experiments were performed according to the standard protocols for Affymetrix GeneChip® Mapping 250K arrays (Gene Chip Mapping 500K Assay Manual (P/N 701930 Rev2)., Affymetrix Inc., Santa Clara, CA).
aCGH was performed using cDNA arrays containing 39,632 cDNAs, representing 22,279 mapped human genes (18,049 UniGene clusters [20], together with 4,230 additional mapped ESTs not assigned to UniGene IDs), according to previously published protocols [21], [22].
The enrichment of phosphopeptides was performed using a gallium-based IMAC column (Pierce, Rockford, IL), according to manufacturer's protocols with minor modification.
