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Dissection of the mouse brain was performed according to coordinates obtained from the mouse brain atlas (Franklin and Paxinos 1997).
Then trepanation of the skullcap was performed according to coordinates obtained from Paxinos and Watson [ 22] (mm from bregma: anterior-posterior = -3.30; lateral = ± 0.0; dorsoventral = -3.8).
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Annotated transcripts overlapping with the selected BACs according to their genomic coordinates (obtained from NCBI Map Viewer version 38; http://www.ncbi.nlm.nih.gov/projects/mapview/) were extracted from the obtained Affymetrix data.
For each gene, genomic sequence around the transcription start site +/- 1 kb according to hg18 coordinates was obtained with the BSgenome R/Bioconductor package version 1.24.0.
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According to,, we obtain (3.9).
According to the coordinates of two distinct spectrum peaks, the rotation angles of their energy-concentrated domain can be determined, and the source number can be easily obtained by the peak number.
According to these coordinates and Eqs.
Velocities of cells were then estimated according to spatial coordinates.
Intron sequences with different AS types were extracted according to the coordinates reported by ASTALAVISTA.
We plotted the samples according to their coordinates along these components.
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