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Graphite furnace atomic absorption spectrophotometry (model 3000; PerkinElmer, Norwalk, CT, USA) was used to quantify blood lead according to a technique described by Miller et al. (1987).
Blood lead measurements were performed using graphite furnace atomic absorption spectrophotometry (model 3000; Perkin-Elmer, Wellesley, MA, USA) at the ABC Hospital Trace Metal Laboratory in Mexico City according to a technique described by Miller et al. (1987).
Blood lead measurements were performed using graphite furnace atomic absorption spectrophotometry (model 3000; PerkinElmer, Norwalk, CT, USA) at the ABC Hospital Trace Metal Laboratory according to a technique described by Miller et al. (1987).
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Malondialdehyde (MDA) levels were evaluated according to a technique previously described [ 20], slightly modified, and quantified by high performance liquid chromatography (HPLC).
The chemotaxis assays were performed according to a technique previously described [ 14].
Surgical therapeutic options included hip disarticulation or a turn-up plasty according to the technique described by Sauerbruch (1922) and by van Nes (1948).
At the same sitting, a metaphyseal osteotomy was performed, according to the technique described by De Bastiani, in preparation for bone transport [14].
A duodenal-preserving resection of the pancreatic head, combined with a longitudinal pancreaticojejunostomy, is performed according to the technique described by Frey and Smith [ 19].
All measurements taken with the goniometer were performed according to the technique described by Norkin and White [23].
Simultaneously, spirometric measurements of tidal volume (VT) and respiratory rate (RR) were performed and, finally, MIP was measured according to the technique described by Marini.
Data analysis was performed according to the technique described by Tichopad et al. and normalized to results obtained with primers specific to GAPDH.
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